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A simple and efficient diffusion technique for assay of endo β-1,4-xylanase activity BJM
Samanta,A.K; Kolte,Atul P; Senani,S; Sridhar,Manpal; Jayapal,Natasha.
Endo-β-1, 4-xylanases is thought to be of great significance for several industries namely paper, pharmaceuticals, food, feed etc. in addition to better utilization of lignocellulosic biomass. The present investigation was aimed to develop an easy, simple and efficient assay technique for endo-β-1, 4-xylanases secreted by the aerobic fungi. Under the proposed protocol, 9 g/L xylan containing agar was prepared in 100 mM phosphate buffer at different pH (4.5, 5.5 and 6.5). The sterilized xylan agar was dispensed in 90 mm petri dishes. 100 µl of culture supernatant of 12 fungal isolates was added to the wells and left overnight at 31±1(0)C. The petri dishes were observed for zone of clearance by naked eye and diameter was measured. Congo red solution (1 g/L)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endo-β-1; 4-xylanase Diffusion technique Congo red assay.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400016
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Prebiotic inulin: Useful dietary adjuncts to manipulate the livestock gut microflora BJM
Samanta,A.K.; Jayapal,Natasha; Senani,S.; Kolte,A.P.; Sridhar,Manpal.
In recent years, there has been a growing appreciation on the relevance of gastrointestinal microflora in both ruminants and non-ruminants owing to revelation of their role in several physiological functions including digestion, nutrient utilization, pathogen exclusion, gastrointestinal development, immunity system, gut gene expression and quality of animal products. The ban imposed on the use of antibiotics and hormones in feed has compelled animal researchers in finding an alternative which could overcome the issues of conventional feed additives. Though the concept of prebiotic was evolved keeping in mind the gastrointestinal flora of human beings, presently animal researchers are exploring the efficiency of prebiotic (inulin) for modulating the gut...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prebiotic; Inulin; Mechanism of action; Livestock.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100001
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